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Superoxide development is actually perceived in the blood and you will BALF with the CMH spin probe. Blood or BALF products had been gathered from PBS- and you can Bleo-treated mice and you can incubated instantaneously that have CMH. The newest samples had been moved to the fresh PTFE tube and you will thumb suspended, and EPR study try collected at the 77 K. The new intensity of nitroxide (CM.) gathered in the bloodstream incubated which have CMH (0.dos mM) from the 37 degrees to own 10 min (Profile 4A).
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